Genetic diversity of two giant freshwater prawn Macrobrachium rosenbergii populations

Twelve microsatellite DNA markers were used to assess the genetic diversity of giant freshwater prawn Macrobrachium rosenbergii of Guangxi population and Guangdong population and to guide the selective breeding work. The results showed that， 12 microsatellite loci in the amplification products of two M. rosenbergii groups were all polymorphic， a total of 71 alleles were amplified， and the average number of alleles per locus was 5.92. The average effective number of alleles， observed heterozygosity， expected heterozygosity， and polymorphism information content of Guangxi population were 3.9183， 0.9505， 0.7376， 0.6558， respectively， while those indexes of Guangdong population were 4.4374， 0.9413， 0.7609， 0.6834， respectively， which had higher genetic diversity than Guangxi population. Results demonstrated that the two populations of M. rosenbergii both had high genetic diversity， which had great potential for the selective breeding of M. rosenbergii.