Separation and purification of polyphenols from areca inflorescence by macroporous resin

Macroporous resins were used to separate and purify polyphenols from the extract of areca inflorescence，and the conditions of separation and purification were determined. The adsorption capacity and desorption rate of six kinds of resins，SP850，XAD-7HP，SP700，D101 and HP2MG，were compared to select the most suitable one，through static adsorption experiment and dynamic adsorption and desorption experiment. The results showed that AB-8 resin was the best one and the kinetic data fitted the pseudo-second-order kinetics model better. Optimal conditions were as follows：the sample pH was 4.0，concentration of ethanol used as elution agent was 60%，initial sample concentration was 0.4 mg/mL，adsorption rate and desorption rate was 3 BV/h. AB-8 resin performed great in separating and purifying polyphenols from areca inflorescence，and the purification of polyphenols was 2.7% at first and increased to 34.6% after purification.