Cloning and expression analysis of cold induced protein kinase gene CIPK07g in rice（Oryza sativa L.）

The gene CIPK07g in rice was cloned，which was one of the members of Ca2+-dependent protein kinase superfamily，and it’s signal peptide，transmemberane domain，conserved domain，expression profile in different organisms and low temperature treatment were analyzed. The results showed that，the cDNA full length of CIPK07g gene was 1 891 bp，including 279 bp 5′UTR and 259 bp 3′UTR，which encoded a polypeptide with 450 amino acids. There was a characteristic conserved domain of CIPK gene family in CIPK07g protein，and the protein was a hydrophilicity protein which included a transmemberane domain but had no signal peptide in N-terminal analyzed by bioinformatic tools. The CIPK07g gene was highly expressed in leaf after 24 h cold treatment.