Separation and purification of flavonoids from bamboo leaves with macroporous resin

This paper used macroporous resin to purify flavonoids from bamboo leaves，discussed the kinetics of absorption and desorption of static and dynamic of macroporous resin to flavonoids，and optimized the process of resin column chromatography dynamic as well. The results showed that D101 macroporous resin was suitable for purification of flavonoids from bamboo leaves；the optimum process conditions were as follows：pH 8.0，flow rate of loading sample at 1 mL/min，the solvents for adsorption 20%，40%，60% and 80% ethanol respectively with 2 BV at the flow rate of 1.5 mL/min. Using the optimized purification process，three flavonoids products with 50.9%，38.0% and 35.8% purity could be obtained，and the total recovery rate of flavonoids was 63%. This process can meet the requirements of high product purity，and ensure the high utilization rate of recycling of bamboo leaves flavonoids，it shows good feasibility.