Research on germplasm in vitro preservation technology of endangered plant Begonia fimbristipula

Taking tube seedlings of endangered plant Begonia fimbristipula as materials，the best way of in vitro preservation of germplasm was studied. The results showed that conventional medium 6-BA and NAA combination was not suitable for germplasm in vitro preservation of B. fimbristipula，the survival rate was only 5% after 90 d. PP333 inhibited the differentiation of tube seedlings sprout plexus，significantly promoted the formation of adventitious roots， after 180 d，only the survival rate of 3.0 mg/L PP333 treatment reached 55%，variation of germplasm in restoring growth. ABA（0.5-2.0 mg/L）inhibited the growth of tube seedlings，after 180 d，the test-tube seedling survival rate was 100%，the somaclone was better after recovery training. 1/2 MS culture medium had the best effect on B. fimbristipula， the survival rate was as high as 85% after 240 d. After restoring growth，the tube seedlings multiplication and their growth were superior to other processings. 1/2 MS culture medium can be used as the best training method for germplasm in vitro preservation of B. fimbristipula.