Identification and biological characteristics of the pathogen of oil tea anthracnose in Hainan
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Graphical Abstract
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Abstract
In order to identify the pathogen of oil tea anthracnose in Hainan,morphological characteristics, pathogenicity test,analysis of molecular sequences and biological characteristics were studied. The results showed that Conidia were oval or cylindrical,measured 13.0-20.3 μm×3.7-6.2 μm. The results based on the phylogenetic analysis of the rDNA-ITS and TUB2 gene showed that the pathogen was in the same branch with Colletotrichum gloeosporioides reference isolates in the phylogenetic tree,whose bootstrap value was 100%. Effects of carbon source,nitrogen source, temperature and pH on pathogen growth were significantly different. The optimal carbon source and nitrogen source for pathogen growth were trehalose and peptone respectively. The optimal temperature and pH value for pathogen growth were 28℃ and 6 respectively. Based on above results,the pathogen of oil tea anthracnose in Hainan was identified as Colletotrichum gloeosporioides(Penz.)Sacc.
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