Detection of β-lactamases-resistant genes in Escherichia coil isolated from ducks

To understand the resistance of β-lactamases-antibiotics in Escherichia coil isolated from ducks， and the situation of carrying β-lactamases-resistant genes，we randomly selected 15 strains E. coil isolated from ill ducks from some scale duck farms in Sansui county of Guizhou province. The drug sensitive test with 8 kinds of β-lactamases-antibiotics was carried out by drug sensitive slips. The β-lactamases-resistant genes of SHV type， CTX-M type and TEM type were detected by PCR， the sequences of drug resistance genes were analyzed and some relevant sequences in GenBank were compared by DNA Star software. Results showed that serious resistance existed in 15 strains of E. coli to 8 kinds of β-lactamases-antibiotics，the resistance rate was 100%. The TEM type and CTX-M type resistant genes were detected，the detection rate were 46.7% and 100%，while the SHV type resistant gene was none visible. There were 7 strains of bacteria with two kinds of drug-resistant genes at the same time. Sequence alignment results showed that the similarity was above 90% between the tested sequences and corresponding resistance gene sequences in the database.