Medium optimization of pectinase production by Aspergillus niger SW06 using response surface method

Using central composite design and response surface methodology, the fermentation condition of pectinase producing strain Aspergillus niger SW06 was optimized. Firstly, the optimum temperature and pH for pectinase production was optimized and the optimal carbon source, nitrogen source and inorganic salts were selected by single factor method. Based on the single factor test, a central composite design was used for regression analysis to determine the optimal culture conditions. The results showed that the optimal temperature and pH were 28益and 5, respectively. The optimal carbon and nitrogen sources were fructose and peptone, respectively. The optimal fermentation conditions were as follows: 4.89 g/100mL fructose, 0.50 g/100mL peptone, 0.60 g/100mL pectin. Under the optimal fermentation conditions, the pectinase activity was up to 4 175.65 U/mL