Damage effects of 1，2，4-TCB on DNA of zebrafish hepatocyte measured by comet assay

In order to explore the DNA damage in cells induced by 1，2，4-TCB，hepatocyte cells of zebrafish cultured in vitro were exposed to different concentrations of 1，2，4-TCB（0，1，5，10 mg/L）for 2 h. Then，the comet assay was applied to measure the DNA damage. Results showed that tail length，tail DNA rate of hepatocyte cells both significantly increased when treated with more than 5 mg/L 1，2，4-TCB compared with the control group. Meanwhile，the DNA damage increased gradually with the increase of 1，2，4-TCB concentration. There was an obvious dose relationship between 1，2，4-TCB concentration and DNA damage（tail length，tail DNA rate，tail moment）. It was concluded that 1， 2，4-TCB could induce DNA damage in hepatocyte cells of zebrafish