Propagating and regeneration for tobacco germplasm resources in Guangdong

Propagating and regeneration for 83 tobacco germplasm resources was counducted in the spring of 2013, including 45 flue-cured tobacco and 38 air-cured tobacco, and the main characteristics of field identification were completed. The results showed that the germination rate of 68 germplasm was in accordance with the requirements of germplasm bank. Large differences were exsited in the growth period, morphological characteristics and main agricultural characters during the germplasm resources. The CV of growth period was 5.7%-16.9% , arranged from longer to shorter as: the day from transplant to budding, the day from transplant to central flower opening, the whole growth period, seedling age. 耶Hongda 11爷had the longest whole growth period (192.0 days), and 耶Jiangyou tobacco爷had the shortest whole growth period (149.0 days). The CV of main agricultural characters was 6.4%-27.1%, arranged in the following order: leaf number, node interval, angle between stem and leaf, plant height, leaf width, corolla diameter, calyx length, leaf length, stem girth, angle between main vein and lateral veins, corolla length. Tobacco mosaic virus (TMV) was the most serious of natural infection in field, followed by bacterial wilt, cucumber mosaic virus (CMV), black shank, potato virus Y (PVY) sporadically occurred. The natural incidence rates of 耶Guangza 87爷, 耶Black leaf No.1爷, 耶Heshan Zhongnan爷, 耶Enzhong Niuli爷were zero.