Screening and identification of endophytic fungi withantinematodal activity against Bursaphelenchus xylophiusfrom Anoectochilus formosanus Hayata

To obtain the fungal strain with nematicidal activity from the endophytic fungi of Anoectochilusformosanus Hayata，endophytic fungi were isolated from A. formosanus by surface disinfection method. Strainsnematicidal activities were assayed in vitro by the soaking method，then the active strains were identified through themorphological characters and molecular systematics. The results showed that 139 strains were isolated from A. formosanustotally and 21 strains had more than 50% of nematicidal activity. Among them，the culture filtrate of strains XX34 andXX49 exhibited the mortality of 100% under 24 h treatment with stable genetic of nematicidal activity. And the strainsXX34 and XX49 were identified as Eurotium amstelodamiand and Xylaria bambusicola，respectively. The resultsindicated that screening of endophytic fungi with efficient nematicidal activity had a great development and applicationvalue in biological control of pine wilt disease.