Construction and screening of RNAi mutant library of rice

143 transgenic plants were obtained by transforming the hpRNA Agrobacterium library into the callus of japonica variety Taipei 309. The detection of 36 transgenic plants showed that the GUS positive was 77.78%，and the sequencing of 35 PCR positive plants were well matched with japonica rice mRNA sequences. 33 materials with mutant phenotype were screened and one lesion mimic mutant was identified from mutant library with 61 T1 materials. The sequence of insertion showed that the gene production belonging to Alpha-amylase inhibitor protein family was RAL6- Seed allergenic protein RA5/RA14/RA17 precursor，it plays an important role in plant's defense system.