Tissue culture and rapid propagation for Oncidium inflorescence shoots

The technology of tissue culture and rapid propagation for Oncidium inflorescence shoots was studiedusing the cut -flowers of Oncidium Gower Ramsey as test material. The results showed that the shoots oninflorescence at maturity level 2 (bamboo shaped appearance, bud and bifurcation pedicel bracts, about 70 cm) andthose in the central section of inflorescence were best suitable as explants. The suitable disinfection time ofinflorescence shoots with 0.1% mercuric chloride solution was 12 min; 1/2MS+6-BA 2.0 mg/L+TDZ 1.0 mg/L+NAA0.2 mg/L was the best original induction medium. Successive transfer cultrue took 6-BA 3.0 mg/L+NAA 0.3 mg/L asthe best hormone combinations and good ideal culture conditions were at 25益and light intensity of 2 500lx (lighttime 10-12 h/d).