Screening and identification of phosphate solubilizing bacteria in the rhizosphere of camellia

Phosphate solubilizing bacteria were isolated from the rhizosphere of camellia袁and six strains were screened by using traditional method of isolation and culture of microorganisms. By using the way of clear zone, phosphate solubilizing bacteria from the rhizosphere soil were preliminarily isolated, and the high effective phosphate solubilizing bacteria were further screened by using molybdenum-antimony anti-spectrophotometric method, strain 3- Y -08 was the most active. The strains and 16S rDNA and phylogenetic tree were identified by studying the morphology, cultural, physiological and biochemical properties. Thus the strain was preliminarily identified as Bacillus megaterium.