Study on genetic diversity of Fusarium mangiferae from mango malformation disease by ISSR analysis

In order to explicit the genetic diversity of 38 strains Fusarium mangiferae from mango malformation disease from Panzhihua, Sichuan, and Huaping, Yunnan in China, the ISSR technique was used. 72 bands were amplified by 14 ISSR primers, with an average of 5.14 bands per primer. The size of the amplified products ranged from 300 bp to 2 000 bp. The genetic similarities among isolates ranged from 0.5972 to 0.9862. Isolates MG16 and MG17 were the most similar(0.9862 genetic similarity), whereas isolates MG07 and MG33 were the least similar(0.5972) and were grouped in separate clusters. At a genetic similarity of 0.76, the unweighted pair -group method with arithmetic mean analysis separated the isolates into five distinct clusters, comprising 33, two, one, one and one isolate, respectively. The ISSR data supported the conclusion that the isolates of F. mangiferae were not necessarily related by geographic origin, type of tissue or the mango cultivar from which they were isolated. This is the first report on the diversity of F. mangiferae species associated with mango malformation in China.