Tissue culture and plant regeneration of Arrhenatherum elatius

The influences of different culture medium on the induced callus, induced buds and roots of Arrhenatherum elatius with the leaves as explants were explored. The results show that the optimum induced callus medium was B5+2,4-D 4 mg/L+CH 0.3 g/L; the optimum induced buds medium was MS+6-BA 5 mg/L+NAA 2 mg/L and the optimum induced roots medium was 1/2MS+6-BA 0.1 mg/L+NAA 1.0 mg/L+0.1% acticarbon.