Establishment of research system for water stress induced gene transcription factors in Brassica oleracea

In this study, the vector of promoter luciferase was built and transfected into kale callus using agrobacterium mediated method. The luciferase gene was confirmed to be integrated into Brassica oleracea genome with luciferase detection through preliminary screening with resistance genes. Gradient NaCl was applied in the processing of callus processing detection. The results showed that in different concentration of NaCl treatments, the expression of luciferase activity increased. Through the TFSEARCH prediction, the study model of transcription factors in plants was established. Combined with existing research results, the mRNA expression of CRP and MADS detected by using real-time quantitative PCR, increased under the stimulation of NaCl.