Liquid fermentation culture medium and process optimization for Hericium erinaceus

The optimum liquid medium of submerged fermentation for Hericium erinaceus was studied in this paper.The single factor experiment results showed the optimum carbon source was soluble starch, the optimum nitrogen source was peptone, the optimum inorganic salt was KH2PO4, and the optimum vitamin was VB1. The homogeneous design experiment results showed that the optimum liquid medium compositions were as follows, soluble starch 4.6%, soybean meal 4.6%, KH2PO4 0.02%, and VB1 18 mg/100mL. The central composite design-response surface methodology results showed that the optimum fermentation process was that inoculums’concentration of 11%, temperature of fermentation at 25.8℃, speed of agitator at 137 r/min.