Cloning and expression analysis of BdPP2C2 gene from Brachypodium distachyon

A PP2C gene named BdPP2C2 was isolated from Brachypodium distachyon, and the result of sequence analysis proved that the length of its ORF was 1 368 bp. Phylogenetic analysis revealed that BdPP2C2 had the closest genetic relationship with AtPP2C56. Quantitative real-time polymerase chain reaction (qRT-PCR) assay reveale that the expression level of BdPP2C2 was induced by ABA, ethylene, H2O2, NaCl and cold treatments. These results indicated that BdPP2C2 was a response factor in the condition of abiotic stresses, and it might be regulated by relevant signaling molecules.