Cl oni ng and codi ng s equenceanal ys i sf or TRAF6 genei n cat t l e

I n or dert o cl ar i f y t he st r uct ur e and f unct i on ofbovi ne TRAF6 gene,t he codi ng r egi on sequence ( CDS)of TRAF6 gene ofdi ar y cat t l e wasi sol at ed by gene cl oni ng t echnol ogy,and i t schar act er ,st r uct ur e and f unct i on wer e anal yzed by bi oi nf or mat i csmet hods .The r esul t sshowed t hat1 803 bp cDNA ( GenBank I D:DQ471669)ofcodi ng sequence ofcat t l e TRAF6 gene wasi sol at ed,whi ch i ncl uded t he compl et e 1 629 bp CDS,coded 542 ami no aci ds ( GenBank I D:ABF06558) , and l ocat ed on many cel lor gans ,i ncl udi ng cel lnucl eus ,cyt opl asm,mi t ochondr i a and endopl asmi c r et i cul um.The mol ecul ar wei ghtand i soel ect r i cpoi ntofdi ar ycat t l eTRAF6 pr ot ei n wer e61. 57176 ku and 6. 09,r espect i vel y.TRAF6 pr ot ei n cont ai ned sever aldomai ns i ncl udi ng RI NG -f i nger ,z f-TRAF and M ATH_TRAF6.The homol ogi es ofTRAF6 pr ot ei n bet ween di ar y cat t l e and human,mouse,r at swer e 89% ,84% and 82% ,r espect i vel y.The homol ogi esofpr ot ei n domai n among al lspeci es ment i oned abovewer e up t o95% .Accor di ngt her esul t sand t hekeyf unct i onsofTRAF6 pr ot ei n f ormanysi gnalt r ansduct i on pat hwaysmedi at ed by TNF/ TLRs / TI R superf ami l i esi n human,i tcoul d i nf ert hatdi ar y cat t l e TRAF6 mi ghtbe an i mpor t ant si gnalmol ecul arf orr egul at i ng manysi gnalt r ansduct i on pat hways ,and mi ghtbe ser ved asver y i mpor t antf unct i onsf ormany bi ol ogi calpr ocesses ,l i kei mmuner esponse,i nf l ammat i on,cel lapopt osi sand di f f er ent i at i on.