Es t abl i s hm entand appl i cat i on ofLAM P m et hod f ordi agnos i ng PCV-2

Accor di ng t o t he gene sequences i n GenBank ofPCV -2 ORF2 gene,t wo pai r s ofspeci f i c pr i mer s wer e desi gned f orampl i f yi ng PCV-2 ORF2 gene.By opt i mi zi ng t he r eact i on condi t i on ofLAM P and sensi t i vi t y t est ,speci f i ci t y t est ,di agnosi ng met hod f orPCV2 wasest abl i shed.The sensi t i vi t y t estshowed t hatt he mi ni mum amountofnucl ei c aci d was 0. 30 pg/ L f orLAM P,whi l e 30 pg/ L f orPCR.Speci f i ci t y t esti ndi cat ed t hatPCV-1,PPV,PRV,PRRSV and CFSV wer e not det ect ed.The det ect i on r esul t sof95 sampl esf r om nat ur ali nf ect i on swi ne i ndi cat ed t hatcoi nci dence r at e ofLAM P met hod t estr esul t s and PCR det ect i on r esul t s was 95. 8% .The ampl i f i cat i on ofLAM P coul d be accompl i shed wi t hi n 30 mi n.And al so t he r esul t s coul d be det ect ed by naked eyes.LAM P i s a si mpl e and r api d met hod f ordi agnosi ng PCV-2,and wwi u meett he needsofr api d quar ant i ne f orgr assr oot sf i el d.