I dent i f i cat i on ofSubgroup 4 M YB t rans cri pt i on f act or- i nt eract i veprot ei nsus i ng yeas tt wo-hybri d s ys t em

M YB t r anscr i pt i on f act or spl ay i mpor t antr ol esi n t r anscr i pt i onal l y r egul at i ng t he synt hesi sofsecondar y cel l wal l .To f ur t heri nvest i gat e t he r ol esofSubgr oup 4 M YB t r anscr i pt i on f act or s i n popl ar ,yeas tt wo-hybr i d syst em wasused t o f i nd outt he i nt er act i ve pr ot ei ns .The r ecombi nant s pDHB1-M YB057,pDHB1-M YB156 orpDHB1-M YB221 pl asmi ds wer e us ed asbai tpl asmi d t o scr een a cDNA l i br ar y const r uct ed f r om Popul oussecondar y vascul art i ssue,and 9 i nt er act i ve pr ot ei ns wer e got t en. The pot ent i al f unct i ons of t he candi dat e i nt er act i on pr ot ei ns wer e anal yzed usi ng bi oi nf or mat i cs met hods.Thi s wor k wi l lhel p t o cl ar i f y t he r ol e ofsubgr oup 4 M YB t r anscr i pt i on f act or si n t he synt hesi s ofsecondar y cel l wal li n pl ant s.