St udy on M YB geneexpres s i on vect orcons t ruct i ng and t rans f orm at i on ofSt yl os ant hesgi anens i scv.Reyan No. 2

The f ul ll engt h ofM YB gene wasampl i f i ed f r om Ant hur i um andr aeanum,t hi s gene was cl oned t o pM D18- T f i r st l y and t hen i nser t ed pBI 121 under CaM V35S pr omot er t o const r uct expr essi on vect or Cam35S-GFP-M YB.t he expr essi on vect or was t r ansf er r ed i nt o EHA 105 by agr obact er i um -medi at ed t r ansf or mat i on,t r ansf or med t he St yl osant hes gi anensi scv.Reyan No. 2 and s ome t r ansgeni c pl ant swasobt ai ned.Taki ng 10 pl ant soft r ansf or med s t yl o f orPCR anal ysi s, t he r esul t s s howed t hat M YB gene successf ul l y i nt egr at ed i nt o t he 6 t r ansgeni c pl ant s.The t r ansgeni c pl ant s made i t possi bl e t o i mpr ove pal at abi l i t y and r esi st ance ofSt yl o,and t o pr ovi de a r el i abl e basi sf orgenet i c t r ansf or mat i on ofSt yl o i n f ut ur e.