Cloning and prokaryotic expression of Musca domestica carboxypeptidase gene

The complete coding sequence (registration number: KF939629.1) of carboxypeptidase (CP) gene screened from cDNA library plasmid of Musca domestica was obtained by PCR conducted with the CP cNA library plasmid as template. The basic physical and chemical properties of the gene and its coding protein, signal peptide and subcellular localization were predicted and analyzed by the bioinformatics methods. Recombinant plasmid PEASY -E1 -CP was constructed and induced expression in E. coli OrigamiB(DE3). The results showed that the CP-ORF was 1 284 bp in length encoding 428 amino acids with a predicted molecular mass of 47.8 ku, pI of 6.10 and conserved domain of carboxypeptidase family. The recombinant plasmid PEASY-E1-CP was induced by IPTG and the protein expressed in E. coli