Effects of phytosterol on growth inhibiting and apoptosis induction of melanoma cells
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Graphical Abstract
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Abstract
Effects on viability and morphology changes of melanoma B16F10 cells induced by stigmasterol and 茁- sitosterol were studied by MTT, inverted phase microscope and fluorescence microscopy. The results indicated that 茁- sitosterol (0.20, 0.30 mmol/L) inhibited the growth of B16F10 cells and significantly showed dose-dependence. Stigmasterol (0.20, 0.25 mmol/L) also inhibited the growth of B16F10, but showed no dose-dependence. Meanwhile, significant apoptosis was induced by stigmasterol and 茁-sitosterol. Identified by the increase of apoptotic bodies and death cells, and the decrease of adherent cells, typical apoptosis appeared in B16F10 cells treated by stigmasterol for 48-72 h. A number of cells in presence of 茁-sitosterol appeared vacuoles and some parts of cells appeared apoptotic bodies. Fluorescence microscopy established that numbers of cells treated by stigmasterol and 茁-sitosterol appeared apoptotic characteristics, like condensed chromosome, swollen nuclear and apoptotic body. Accordingly, stigmasterol and 茁-sitosterol partly inhibited B16F10 cells growth through inducing the cells apoptosis.
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