Study on establishment of transformation system mediated by Agrobacteriume in Actinidia chinensis Planch.cv Huayou.

Young leaves of plantlets were used as explants to investigate the factors influencing plants regeneration & transformation efficiency of Actinidia chinensis Planch.cv Huayou. The results showed that in medium plus 0.75~1.0 mg/L thidiazuron (TDZ), callus formation rate reached 85.7%-90.1% and differentiation of buds rate reached 41.8% to 44.6%. Relatively high callus formation rate ( 96.7%-100 %) & differentiation of buds rate ( 67.4%-71.9 %) were achived. when leaf explants were grown in medium containing 1.0 mg/L TDZ and 0.1-0.4 mg/L IAA. GUS Transient expression rate of explants & anti -Kan adventitious buds was significantly enhanced while 75 -100 mol/L AS was supplied in the Agrobacterium infecting cultures & co-cultivation medium. It was useful to increase GUS Transient expression rate of explants & anti-Kan adventitious buds that explants were precultured for 2-3 days before infected by Agobacterium. All regenerated plantlets were deemed putative transgenic by histochemical GUS assay and PCR detection.