Establishment of PCR testing technique for citrus HLB in Zhaoqing and Yunfu

The experiment was made with citrus leaves as samples and by means of DNA extraction. Specific primers from HLB agents were applied to amplify through PCR, the final product of which would be purified and recollected. 16Sr DNA segments were positively cloned and sequenced. A PCR testing technique was established for citrus HLB in Zhaoqing and Yunfu area. The PCR technique was applied to test suspected citrus HLB samples all cross Zhaoqing and Yunfu. Experiment results indicated that PCR testing technique was feasible and citrus plants with HLB infection were discovered in Zhaoqing and Yunfu.