Preparation of directional release lactic acid bacteria microcapsule

In order to provide a new method for large-scale production of lactic acid bacteria microcapsule, acid resistant and directional release microcapsule of Lactobacillus paracasei R8 was prepared by screening the cryoprotectant and prebiotics, optimizing the embedding method, and using spray method to solidify the microcapsule. The optimal cryoprotectant was whole egg liquid, and the optimal prebiotic was inulin. Microcapsule preparation conditions were as following spray pressure was 0.2 ~0.3 MPa, embedding liquid flow rate was 30 mL/min. The optimal composition of embedding liquid was bacteria sludge 5%, inulin 2%, eggs 20%, sodium alginate 1%. The optimal composition of solidifying liquid was chitosan 0.5%, CaCl2 2.5%, acetic acid 1%.