Clone of rice blast resistance gene Pik2-H4 and it爷s interaction protein screening

The rice blast broad spectrum resistance gene Pik2-H4 was cloned from the highly resistance lines H4. The coding sequence of Pik2-H4 is 3 066 bp, which is different from the corresponding allele sequence of Nipponbare. Pik2-H4 belongs to NBS-LRR genes and codes a protein containing NB-ARC domain and leucine-rich repeats. To find the interacting proteins with Pik2-H4 from the rice, the yeast two-hybrid system was used and three important protein (LOC-Os08g39300, LOCOs03g25960 and LOC-Os09g29130冤were identified. The results could provide some new information for the mechanism of blast resistance signal pathway induced by the Pik2-H4.