Tissue culture and rapid propagation of Grevillea lanigera

This paper took the preliminary trial on tissue culture and rapid propagation of Grevillea lanigera. The results showed that the contamination rate of explants could be controlled below 28.1% through the method of twice disinfection using mercuric chloride (0.025% and 0.1%). According to the results, the optimum medium for bud induction and multiplication was WPM+6-BA 1.0 mg/L+IBA 0.2 mg/L+sucrose 30 g/L; the suitable subculture cycle was 20 d; and the proliferation multiples of adventitious buds reached 1.65. The optimum medium for root induction was 1/2WPM+IBA 0.6 mg/L+sucrose 15 g/L and 91.7% of rooting percentage was obtained. The plantlet survival rate could exceed 90%.