Study on the identification and activity of chitinase produced by Brevibacillus laterosporus

The strain GWM1.8072 was isolated from the soil. The results showed that strain GWM1.8072 was found to have strong chitosanase activity. Through study on its morphological, physiological and biochemical characteristics as well as 16S rDNA sequence homology comparison, the strain GWM1.8072 was identified as Brevibacillus laterosporus, which was almost closest relative to the standard strain with more than 99.7% similarity in sequence under the phylogenetic tree.Through the preliminary detection of the fermentation product analysis, enzyme activity of GWM1.8072 reached 4.52 U/mL,and the experimental results showed that this strain GWM1.8072 had strong chitinase activity and characteristics of biological control袁which had a prospect of biological control application.