Construction and promotion research of MSTN reporter plasmid in Guanling cattle

The MSTN promoter was amplified and inserted into luciferase pGL3-Basic vector to construct recombined pGL3-Basic- MSTN -promoter reporter plasmid. Transient transfection was performed in mouse myoblasts cell line C2C12 and mouse embryonal fibroblast cell line 3T3-L1, and pRL-TK was used to determine the transfection efficiency. The sequencing results confirmed that the pGL3-Basic-MSTN-promoter sequence was correct and expression in C2C12 and 3T3-L1 were 14.53 times and 5.02 tines more than pGL3 -Basic plasmid respectively. The results could lay an experimental foundation for further studying the mechanism of MSTN expression and regulation.