Dissociation and culture of ear marginal fibroblasts in Tibetan pigs

The study was to isolate and culture fibroblast cell lines of ear marginal tissues in Tibetan pigs by direct adherent culture methods in vitro. The results showed that fibroblasts were successfully isolated and could be used for passage and freezing; the cells could still be cultured and subcultured after freezing-thawing, both of the survival rates of cells before and after freezing were above 90%; the growth curve was typical of S type, the incubation period was 0-2 d, the logarithmic growth phase was 3-9 d, and the plateau was 10-12 d after the cells inoculation. The ear fibroblasts in Tibetan pigs were successfully cultured. The result of this study could lay the foundation for research on somatic cell cloning and breed conservation of Tibetan pigs.