Systematic position of Dendrobium huoshanense inferred from ITS、Nad intron2 and psbA-trnH DNA sequences

Flora Republicae Popularis Sinicae (Vol.19) described Dendrobium officinale, D.moniliforme，D.huoshanense and D. tosaense as the four independent species. However, in Flora of China (2009 edition), D.officinale, D.huoshanense and D.tosaense was the synonym of D.catenatum. In this paper, the sequences of ITS, Nad intron2 and psbA -trnH of the above four Dendrobium species were analysed, the results show that there was little variance of base sequences between D. officinale and D.tosaense in ITS ，Nad intron2, and psbA-trnH. There were significant differences between D.huoshanense and D.officinale or D.tosaense in ITS, Nad intron2 and psbA-trnH， but little differences between D.huoshanense and D.moniliforme. Their genetic distance between D.moniliforme and D.huoshanense was smaller than that between D.huoshanense and D.tosaense. The above results suggested that D.huoshanense should be the synonym of D.moniliforme.