Effects of basal mediums on callus induction and plant regeneration of Eucalyptus urophylla ×伊E. grandis

The callus induction and shoot regeneration were studied systematically in elite clone of Eucalyptus urophylla ×E. grandis by comparing the basal mediums. The results showed that sterile clones of seedling stem segments were used as explants and cultured in a MS medium supplemented with the synthetic growth regulator 2 mg/L PBU and 0.05 mg/L IAA. After cultivation for 5 d, 96.3% of explants formed callus. Callus were transferred to MS medium containing 1 mg/L 6-BA and 0.05 mg/L NAA to induce bud formation, and 91.8% of the callus induced by PBU exhibited adventitious bud formation. Effect of basic mediums on the adventitious shoot proliferation and development from hypocotyls were greatly significant differences.