Tissue culture and rapid propagation of Cyphomandra betacea

Taking buds of plantlets as material, effects of basic mediums, hormone density matching on inducing bud, bud proliferation,elongation and rooting were studied. Influence of moisture, temperature, substrate on the survival rate of plantlets transplanted were tested，too. The results showed that the medium of MS+6-BA 3.0 mg /L+NAA 0.01 mg/L+sucrose 3% was conducive to induce bud formation, MS+6-BA 2.0 mg/L+NAA 0.01 mg/L+sucrose 3% was conducive to bud proliferation, MS+6-BA 0.5 mg /L+ sucrose 3% was conducive to elongation, 1/2MS+NAA 0.2 mg/L+ sucrose 1.5% was conducive to rooting. It could improve the survival rate more than 80% of plantlets transplanted by using the matrix consisting of peat, coco peat and perlite in late spring and autumn.