Purification of DndB protein involved in DNA sulfur modification

According to the character of DndB expression vector, an appropriate purification system consisting of Ni2+-NTA affinity chromatography, Source Q anion-exchange chromatography and Superdex 200 gel filtration chromatography was built to purify DndB protein with high purity for structure biology and enzymology study. This work laid a foundation for functional study of DndB protein and mechanistic study of DNA sulfur modification.