Confirmation of the virus infecting and causing the Hibiscus rosa-sinensis yellow leaf curl disease in Guangdong

According to the conserved sequence of coat protein genes of geminivirus, a pair of degenerate primers was designed and used to detect the presence of CLCuMV. 100 Hibiscus rosa-sinensis yellow leaf curl disease samples were collected from 20 sampling points in Guangzhou, Zhongshan, Zhuhai, Dongguan and Shenzhen. Detection was done by PCR technology, and then PCR products were cloned into the vector pGM-T. Sequence analysis indicated that the nucleotide sequences of the amplified products from above five cities had 97.5% identity, and shared 99% sequence identity with CLCuMV, which indicating the virus infected the hibiscus was CLCuMV. The DNA specific sequence was presented in 78 samples from 19 sampling points, and the positive rates in all samples and regions were high with 78% and 95%. That meant Hibiscus rosa-sinensis yellow leaf curl disease was common in Guangdong.