Effect of rooting powder on in vitro shoot rooting and transplanting seedling quality of sugarcane

Increase in sugarcane transplanting survival rate and improvement in temporary planting seedling quality were achieved through enhancing the quality of tissue culture plantlet root. In order to study the influence of rooting powder on sugarcane in vitro shoot rooting and transplanting seedling quality, difference concentrations of rooting powder were added into the rooting media. The results show that: Extremely significant levels were observed in sugarcane tissue culture plantlet rooting, transplanting survival rate and temporary planting seedling quality when rooting media were supplemented with 25~200 mg/L of rooting powder. In particular, with rooting powder of 100 mg/L, plantlet root quality, transplanting survival rate and temporary planting seedling quality are the best. At the same time, many long and thick roots of seedlings in the bottles are achieved which resulted in tall, stout, and colour dark green temporary planting seedlings with many new long thick white roots and that are suitable for field transplanting with high survival rate.