In vitro micropropagatiing of apical meristem and seed breeding to the 10th Ningde-sweet potato
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Graphical Abstract
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Abstract
To keep the good character of 10th Ningde-sweet potato, extend the seed application experience in field, virus-free plantlets was obtained by apical meristem culture and indicator plant detection method. The experimental results showed that the first culture medium for apical meristem growth of the 10th Ningde-sweet potato was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, seedling rate up to 94.4%, and subculture medium for stem segments propagating was 6-BA 0.2 mg/L+NAA 0.05 mg/L, the propagation coefficient was up to 5 times. The yield of its virus-free seedling increased by 16.5% than that of the control original varieties, reached extremely significant level, so it could be popularized in large area.
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