A new mathematic model of vehicle routing problem for the fresh agricultural products in cold-chain was provided in this paper, which was based on the analysis of the energy consumption of the refrigerator car. Ant colony algorithm and MATLAB tools were applied into an example to calculate and analyze this method. The result showed that this new model was more suitable for the fresh agricultural products to select the optimal paths in cold chain.[J]. Guangdong Agricultural Sciences, 2013, 40(9): 178-181.