Study on ITS sequences of four kinds of mangrove plants in Qiao island

The genomic DNA of the four kinds of mangrove plants were extracted from leaves by an improved CTAB method, the ITS sequences were amplified by PCR with universal primers and the PCR products were direct sequenced. The length of the ITS1 of four kinds of mangrove plants were 404 bp, 362 bp, 460 bp and 439 bp, respectively; the length of the ITS2 were 219 bp, 208 bp, 231 bp and 226 bp,respectively. Submitted the results of the ITS sequences of four mangrove species to GenBank, then the Registry Number obtained.