Effects of amino fat agent prepared by waste sile and cocoon on the growth of Chlorlla spp.

Silk reeling frigon contains large amounts of waste sile and cocoon protein, through hydrolysising, we can get amino acid hydrolysate, in which the free amino acid N can provide abundant source for the growth of chlorella. Taking chlorella biomass as indicators, the paper used single factor experiment to study the effect of some factors inclding different concentrations of amino acid hydrolyzate, K2HPO4, MgSO4, Na2EDTA, photosynthetic bacteria and bacillus on the growth of chlorella. This paper prepared an amino acid fertilizer agent using waste sile and cocoon as raw material, the formula was following: 10 mL amino acid hydrolysate which pH 7.0~7.2 and the concentration of 83.33%; 0.8 g K2HPO4, 2.0 g MgSO4`7H2O, 0.025 g Na2EDTA, 1.02*10^4 CFU photosynthetic bacteria:5.4*10^4CFU bacillus=1：1 in each 200 mL fat agent.