Effect of EMS treatment on phenotype and genetic of Loquat plantlet

The stem tips of test tube plant clone were used for EMS chemical mutation in the study, which were grown from young embryo of 'Da Wu Xing' loquat, the results were as follows: 0.1%~0.9% EMS soak 0.5~1.5 h, the death rate of the test tube seedlings was low; by 0.1%, 0.5 h EMS treatment, the average plantlet height and chlorophyll content were remarkably higher than those of contrast, the chlorophyll concentration was 4.21 mg/g; the RAPD analysis showed that amplified spectrum of some plants changed compared with the contrast.