Tissue culture and hypericin production of Hypericum perforatum L.

In this study, the seeds of Hypericum perforatum were used as the materials. Tissue culture seedlings rooting culture experiment was carried out to screen and optimize the rooting culture medium. Then the adventitious roots were induced and cultured in bioreactor. The effects of sucrose concentration, NH4+/NO3- ratio and MS strength on hypericin content of adventitious roots in bioreactor culture were investigated. The results showed that tissue culture seedlings rooted best with IBA 2.5 mg/L, sucrose 30 g/L and agar 8 g/L (pH 5.8) in 3/4MS medium. 40 g/L of sucrose concentration was conducive to the accumulation of hypericin, the hypericin content was highest (0.94 mg/g). When NH4+/NO3- ratio was 30/30, hypericin content and productivity reached the maximum, the values were 0.98 mg/g and 12.41 mg/L respectively. Adventitious roots could grew in different MS strength medium, hypericin produced the largest amount (15.70 mg/L) in 2MS strength.