Inhibitory activity of Shisandra chinensis and Polygonatum sibiricum on Vibrio parahaemolyticus and its biomembrane

The inhibitory effects of Shisandra chinensis and Polygonatum sibiricum on Vibrio parahaemolyticus were tested by disc agar diffusion，and the minimal inhibitory concentration（MIC） and minimal bactericidal concentration（MBC） were detected by double dilution；the effects of S. chinensis and P. sibiricum on V. parahaemolyticus biomembrane were measured by MTT assay. The two species of Chinese herbal medicine had different degree of inhibition. S. chinensis had inhibitory effects with the diameter of inhibition zone（DIZ） of 18.67 （±0.2） mm，and the MIC and MBC respectively were 1.56，3.125 mg/mL；P. sibiricum had inhibitory effects with the diameter of inhibition zone（DIZ） of 11.26（±0.6） mm，and the MIC and MBC respectively were 1.56， 3.125 mg/mL. Both S. chinensis and P. sibiricum exhibited a potent inhibitory activity against V. parahaemolyticus biomembrane formation，when the concentration of medicine was over 3.125 mg/mL.