Objective The study was carried out to clear and definite that the addition of efficient and safe auxiliary N, N-Dimethylformamide (DMF) can improve the sterility of quinestrol baits, and improving the palatability of quinestrol baits to mice.
Method The mice were divided into three groups C, T1 and T2 fed with blank bait, quinestrol bait and DMF-quinestrol bait, respectively, and the bait consumption was calculated. The male and female mice of three treatment groups were caged for 7 days, and the pregnancy and delivery of the female mice were recorded. The high-performance liquid chromatography used to detection the content of quinestrol in brown rice in the different formulations of baits.
Result Both the T1 and T2 groups had significant effects on the body weight of male and female mice. The wet weights of the kidney organs in the T1 and T2 groups were 16.4% and 19.7% lower than the C group, respectively. Quinestrol had significant inhibitory effect on the reproduction of female mice, with the reproductive rate of female mice in T1 and T2 groups decreased to 37.5% and 25.0% of group C, and the number of litters was only 35.0% and 11.1% of group C, respectively. DMF did not significantly improve the palatability of quinestrol drug bait, but the results of high-performance liquid chromatography showed that the content of quinestrol in brown rice in the T2 group was 1.5 times higher than that in the T1 group. DMF increased the permeability of quinestrol in brown rice, thereby increasing the drug intake of mice.
Conclusion This study shows that DMF can effectively dissolve quinestrol and enhance its permeability in brown rice, improving the contraceptive effect of quinestrol drug bait, and has the potential to be developed into a promising contraceptive formulation.
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