Construction of Genetic Linkage Map of Plum (Prunus salicina L.) with ISSR and SRAP Markers

Objective  Plum (Prunus salicina L.) is a tree species with great economic prospect in northeast China, and it has important social and economic values. Genetic linkage maps of plum were constructed by molecular markers to provide a theoretical basis for breeding new plum varieties.

Method  Jilin 6 and Longyuan Qiuli were used as the mapping parents. The authenticity of F₁ progeny was identified by SSR primers. 14 ISSR and 16 SRAP primers were selected to construct the genetic linkage map of plum by using Join Map 4.0 software and "double pseudo-testcross" theory.

Result  In this study, 72 authentic F₁ progeny were obtained. ISSR and SRAP primers produced 5 segregation patterns and 120 marker loci in this F₁ population. A genetic linkage map with 16 linkage groups and 38 marker loci with a total length of 528.5 cM was constructed by software analysis. The groups ranged in size from 9.6-71.1 cM, and the average size was 13.9 cM. The marker had a maximum genetic distance of 52.8 cM and a minimum genetic distance of 1.4 cM.

Conclusion  The construction of genetic linkage maps enriched the genetic research basis of plum in cold region, and contributed to the study of QTL mapping, marker-assisted breeding and gene mapping.