Objective On the basis of alkaline extraction of mulberry leaf protein, microwave-assisted extraction method was used to explore the effects of leaf protein extraction from mulberry leaves under different conditions.
Method The extraction yield of mulberry leaf protein was taken as the index. Based on the single factor test, the process parameters of extracting mulberry leaf protein were optimized by response surface test.
Result With the gradual increase of the pH value of the extraction solution, the extraction yield of mulberry leaf protein increased. When the pH value of the extraction solution was 13, the extraction yield of mulberry leaf protein reached the peak. With the decrease of the solidliquid ratio, the extraction yield of mulberry leaf protein increased obviously, and reached the peak when the solid-liquid ratio reached 1:100 g/mL. With the extension of microwave extraction time, the extraction yield of mulberry leaf protein increased first and then decreased, and reached the peak at 80 s. The extraction yield of mulberry leaf protein increased significantly with the increase of microwave power and reached the peak at 500 W.
Conclusion The optimal extraction parameters of mulberry leaf protein by microwave-assisted alkaline extraction were as follows: pH 11.4, solid-liquid ratio 1:102 g/mL, microwave power 500 W, and microwave extraction time 82 s. Under these conditions, the extraction yield of mulberry leaf protein was 35.84%, which was close to the predicted value of the model. This method greatly reduced the extraction time of mulberry leaf protein and provided a basis for further study and utilization of mulberry leaf protein.
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