Study on Selenium-enriched Cultivation of Ganodermal ucidum in the Artificial Intelligence Climate Chamber

Objective The cultivation methods of selenium-enriched Ganoderma lucidum were studied in the artificial intelligence climate chamber in order to provide references for the commercial cultivation of G. lucidum and the research and development of selenium-enriched products.

Method With selenium ore powder as selenium source and G. lucidum as selenium-enriched carrier, the selenium-enriched G. lucidum was cultivated by solid substrate cultivation method, and a comprehensive study was conducted combined with the polysaccharide and total triterpene contents.

Result Among the six treatments, each solid substrate bacteria package was added with 0 (CK), 1 000, 2 000, 3 000, 4 000 and 5 000 μg selenium, and the selenium contents in spore powder were 0.13, 1.10, 3.20, 4.40, 6.80 and 7.70 μg/g, with the seenrichment coefficients of 1, 8.5, 24.6, 33.8, 52.3 and 59.2, respectively. The selenium contents of G. lucidum fruit body were 0.35, 1.30, 5.15, 10.90, 16.25 and 17.05 μg/g, with the se-enrichment coefficients of 1, 3.7, 14.7, 31.1, 46.4 and 48.7, respectively.

Conclusion There was positive correlation between the selenium contents in spore powder and fruit body, which increased with the increase of selenium in the solid substrate bacteria package. Among the six treatments, there was no significant difference in G. lucidum spore powder and fruit body production, as well as in polysaccharide and total triterpene contents.